1Yarmouk University, Department of Sports/Movement Sciences, Irbid, Jordan
Effect of β-Alanine Supplementation on Repeated Sprint Ability and Responses of Blood Lactate and Bicarbonate in Male Soccer Players
This study was designed to investigate the effect of β-alanine supplementation on sprint time during repeated sprint ability test and blood lactate and bicarbonate responses to the test. Eighteen male soccer players were randomly divided into two groups (β-alanine, n=9 (24.31±2.14 yrs) or placebo, n=9 (23.98±2.07)). We conducted a randomized, double-blind, parallel-group, placebo-controlled study in which participants ingested 4.8 g/day for four weeks of a β-alanine supplement or a placebo. Athletes completed seven repetitions of 30 m interspersed with 30 s recovery intervals. The test was performed before and after four weeks of supplementation. Blood samples were collected from each participant in both groups before and after the test, pre- and post-supplementation to measure lactate and bicarbonate levels. Data showed that the sixth and seventh repetitions were significantly faster after β-alanine supplementation than the placebo (sixth repetition: 3.74±0.04 s vs 3.91±0.09 s, seventh repetition: 3.91±0.07 s vs 4.12±0.14 s, p=0.001, p=0.002, respectively). Before supplementation, however, no differences existed between groups for any sprint time in all repetitions (p>0.05). Data revealed significantly higher lactate concentration in the β-alanine than the placebo after the finish of the test at both pre-supplementation (p=0.022), and post-supplementation (p=0.017). No differences noted between groups in bicarbonate at all measured points. In conclusion, β-alanine supplementation has a beneficial effect on repeated sprint performance in soccer players, probably due to effective vasodilatation mechanism.
carnosine, fatigue, hydrogen ion, fast-twitch fiber, glycolysis
Al-horani, R.A., & Alzoubi, R. (2017). Effect of seven days of beta-alanine supplementation on cycle ergometer wingate test performance. Int J Coach Sci, 11(2), 45-59.
Baguet, A., Koppo, K., Pottier, A., & Derave, W. (2010). β-Alanine supplementation reduces acidosis but not oxygen uptake response during high-intensity cycling exercise. Eur J Appl Physiol, 108, 495-503.
Bate-Smith, E. C. (1938). The buffering of muscle in rigor; protein, phosphate and carnosine. J Physiol, 92, 336-343.
Bellinger, P.M., & Minahan, C.L. (2016). Metabolic consequqnces of β-alanine supplementation during exhaustive supramaximal cycling and 4000-m time-trial performnace. Appl. Physiol. Nutr. Metab., 41, 864-871.
Brisola, G.M.P., Artioli, G.G., Papoti, M., & Zagatto, A.M. (2016). Effects of four weeks of β-alanine supplememntation on repeated sprint ability on water polo players. PLoS ONE, 11 (12), 1-13.
Claus, G.M., Redkva, P.E., Brisola, G.M.P., Malta, E.S., de Poil, R.D., Miyagi, W.E., & Zagatto, A.M. (2017). Beta-Alanine supplementation improves throwing velocities in repeated sprint ability and 200-m swimming performance in young water polo players. Pediatric exercise Science, 29 (2), 203-212.
Danaher, J., Gerber, T., Wellard, RM, & Stathis, C.G. (2014). The effect of beta-alanine and NaHCO3 co-ingestion on buffering capacity and exercise performance with high-intensity exercise in healthy males. Eur J Appl Physiol, 114 (8), 1715-1724.
de Salles Painelli, V., Roschel, H., de Jesus, F., Sale, C., Harris, R.C., Solis, M.Y., Benatti, F.B., Gualano, B., Jr, AHL, & Artioli, G.G. (2013). The ergogenic effect of beta-alanine combined with sodium bicarbonate on high-intensity swimming performance. App. Physiol. Nutr. Metab., 38, 525-532.
Derave, W., Ӧzdemir, M.S., Harris, R.C., Pottier, A., Reyngoudt, H., Koppo, K., Wise, JA, & Achten, E. (2007). β-Alanine supplementation augments muscle carnosine content and attenuates fatigue during repeated isokinetic contraction bouts in trained sprinters. J Appl Physiol, 103, 1736-1743.
Devrnja, A., & Matkovic, BR (2018). The effects of a soccer match on muscle damage indicators. Kinesiology, 50, 112-123.
Dutka, T.L., & Lamb, G.D. (2004). Effect of carnosine on excitation-contraction coupling in mechanically-skinned rat skeletal muscle. J Muscle Res Cell Motil, 25 , 203-213.
Gallant, S., Semyonova, M., & Yuneva, M. (2000). Carnosine as a potential anti-senescence drug. Biochemistry, 65, 866-868.
Glenn, J.M., Gray, M., Stewart, R., Moyen, N.E., Kavouras, S.A., Dibrezzo, R., Turner, R., & Baum, J. (2015). Incremental effects of 28 days of beta-alanine supplementation on high-intensity cycling performance and blood lactate in masters female cyclists. Amino Acids, 47, 2593-2600.
Harris, R.C., Tallon, M.J., Dunnett, M., Boobis, L., Coakley, J., Kim, H.J., Fallowfield, JL, Hill, CA, Sale, C., & Wise, JA (2006). The absorption of orally supplied beta-alanine and its effect on muscle carnosine sunthysis in human vastus lateralis. Amino Acids, 30 (3), 279-289.
Hill, C.A., Harris, R.C., Kim, H.J., Harris, B.D., Sale, C., Boobis, L.H., Kim, C.K., & Wise, JA (2007). Influence of beta-alanine supplementation on skeletal muscle carnosine concentrations and high intensity cycling capacity. Amino acids, 32 (2), 225-233.
Hobson, R.M., Saunders, B., Ball, G., Harris, R.C., & Sale, C. (2012). Effects of β-alanine supplementation on exercise performance: a meta-analysis. Amino Acids, 43 , 25-37.
Hoffman, J.R. (2010). Creatine and β-alanine supplementation in strength/power athletes. Current Topics in Nutraceutical Research, 8 (1), 19-32.
Kendrick, I.P., Harris, R.C., Kim, H.J., Kim, C.K., Dang, V.H., Lam, T.Q., Bui, T.T., Smith, M., & Wise, JA (2008). The effects of 10 weeks of resistance training combined with beta-alanine supplementation on whole body strength, force production, muscular endurance and body composition. Amino Acids, 34 (4), 547-554.
Lancha Junior, A.H., de Salles Painelli, V., Saunders, B., & Artioli, G.G. (2015). Nutritional strategies to modulate intracellular and extracellular buffering capacity during high-intensity exercise. Sports Med, 45 (Suppl 1), S71-S81.
Ririe, D.G., Roberts, P.R., Shouse, M.N., & Zaloga, G.P. (2000). Vasodilatory actions of the dietary peptide carnosine. Nutrition, 16, 168-172.
Sale, C., Saunders, B., Hudson, S., Wise, J.A., Harris, R.C., & Sunderland, C.D. (2011). Effect of β-alanine plus sidium bicarbonate on high-intensity cycling capacity. Medicine and Science in Sports and Exercise, 43 (10), 1972-1978.
Smith, A.B., Walter, A.A., Graef, J.L., Kendail, K.L., Moon, J.R., Lockwood, C.M., Fukuda, D.H., Beck, T.W., Cramer, J.T., & Stout, J.R. (2009). Effects of β-alanine supplementation and high-intensity interval training on endurance performance and body composition in men; a double-blind trial. Journal of the International Society of Sports Nutrition, 6 (5), 1-9.
Suzuki, Y., Ito, O., Mukai, N., Takahashi, H., & Takamatsu, K. (2002). High level of skeletal muscle carnosine contributes to the latter half of exercise performance during 30-s maximal cycle ergometer sprinting. The Japanese Journal of Physiology, 52 (2), 199-205.
Sweeney, K.M., Wight, G.A., Brice, A.G., & Doberstein, S.T. (2010). The effect of β-alanine supplementation on power performance during repeated sprint activity. J Strength Cond Res, 24 (1), 79-87.
Tobias, G., Benatti, F.B., de Salles Painelli, V., Roschel, H., Gualano, B., Sale, C., Harris, R. C., Jr, A. HL, & Artioli, G.G. (2013). Additive effects of bets-alanine and sodium bicarbonate on upper-body intermittent performance. Amino Acids, 45, 309-317.
Varley, M.C., & Aughey, R.J. (2013). Acceleration profiles in elite Australian soccer. International Journal of Sports Medicine, 34 (1), 34-39.
Zhen-He, H., Botinelli, R., Pellegrino, M., & Reggani, C. (2000). ATP consumption and efficiency of human muscle fibers with different myosin isoform composition. Biophys J, 79, 945-961.